Molecular Pathology, Vol 51, Issue 3 138-142, Copyright © 1998 by Journal of Clinical Pathology

Cytochrome P450 1B1 mRNA in the human central nervous system

CR Rieder, DB Ramsden and AC Williams
Department of Neurology, University of Birmingham, Queen Elizabeth Hospital, Edgbaston, UK.

AIMS: To study the expression of CYP1B1 in a variety of human and rat cell lines as a means of identifying a new tool for the investigation of gene regulation. In addition, to identify the expression of cytochrome P450 1B1 (CYP1B1) in different regions of the central nervous system (CNS). METHODS: Reverse transcription-polymerase chain reaction followed by cloning and sequencing were used to detect the expression of CYP1B1 in human cell lines. Poly A+ mRNA from the human spinal cord and from different brain regions was analysed using a CYP1B1 probe labelled with 32PdCTP. RESULTS: Expression of CYP1B1 was shown in a human astrocytoma cell line (MOG-G-CCM). CYP1B1 mRNA was expressed in a variety of regions of the CNS but with a distinct regional specificity. Expression was highest in the putamen. CONCLUSIONS: The expression of CYP1B1 in a human astrocytoma enables this cell line to be used in further studies of regulation and function of this gene. The demonstration that CYP1B1 mRNA is expressed in a variety of regions of the CNS suggests a role for this gene in brain and spinal cord metabolism. The regional specificity of expression might explain the focal damage of certain human neurodegenerative diseases.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Sissung, T. M., Price, D. K., Sparreboom, A., Figg, W. D. (2006). Pharmacogenetics and Regulation of Human Cytochrome P450 1B1: Implications in Hormone-Mediated Tumor Metabolism and a Novel Target for Therapeutic Intervention. Mol Cancer Res 4: 135-150 [Abstract] [Full Text]  
• Maecker, B., Sherr, D. H., Vonderheide, R. H., von Bergwelt-Baildon, M. S., Hirano, N., Anderson, K. S., Xia, Z., Butler, M. O., Wucherpfennig, K. W., O'Hara, C., Cole, G., Kwak, S. S., Ramstedt, U., Tomlinson, A. J., Chicz, R. M., Nadler, L. M., Schultze, J. L. (2003). The shared tumor-associated antigen cytochrome P450 1B1 is recognized by specific cytotoxic T cells. Blood 102: 3287-3294 [Abstract] [Full Text]  
• Gibson, P., Gill, J. H., Khan, P. A., Seargent, J. M., Martin, S. W., Batman, P. A., Griffith, J., Bradley, C., Double, J. A., Bibby, M. C., Loadman, P. M. (2003). Cytochrome P450 1B1 (CYP1B1) Is Overexpressed in Human Colon Adenocarcinomas Relative to Normal Colon: Implications for Drug Development. Molecular Cancer Therapeutics 2: 527-534 [Abstract] [Full Text]  
• Muskhelishvili, L., Freeman, L. D., Latendresse, J. R., Bucci, T. J. (2002). An Immunohistochemical Label to Facilitate Counting of Ovarian Follicles. Toxicol Pathol 30: 400-402 [Abstract]  
• Muskhelishvili, L., Thompson, P. A., Kusewitt, D. F., Wang, C., Kadlubar, F. F. (2001). In Situ Hybridization and Immunohistochemical Analysis of Cytochrome P450 1B1 Expression in Human Normal Tissues. J. Histochem. Cytochem. 49: 229-236 [Abstract] [Full Text]