The control of ccn2^* (ctgf) gene expression in normal and scleroderma fibroblasts

A Leask¹, S Sa¹, A Holmes², X Shiwen², C M Black² and D J Abraham²

¹ Fibrogen Inc, 225 Gateway Blvd, South San Francisco CA 94080, USA
² Center for Rheumatology, Royal Free and University College Medical School, Rowland Hill Street, London NW3 2PF, UK

Correspondence to:
Dr Leask aleask{at}fibrogen.com

Although the role of transforming growth factor ß (TGFß) in initiating fibrosis is well established, the role that TGFß plays in maintaining fibrosis is unclear. The gene encoding connective tissue growth factor (ccn2; ctgf), which promotes fibrosis, is not normally expressed in dermal fibroblasts unless TGFß is present. However, in dermal fibroblasts cultured from lesional areas of scleroderma, ccn2 (ctgf) is expressed constitutively. The contribution of several elements in the ccn2 (ctgf) promoter to basal and TGFß induced ccn2 (ctgf) expression in normal and scleroderma fibroblasts has been investigated. A functional SMAD binding site in the ccn2 (ctgf) promoter that is necessary for the TGFß mediated induction of this gene has been identified. The previously termed TGFß responsive enhancer (TGFßRE) in the ccn2 (ctgf) promoter has been found to be necessary for basal promoter activity in normal fibroblasts. The SMAD element is not necessary for the high ccn2 (ctgf) promoter activity seen in scleroderma fibroblasts. However, mutation of the previously termed TGFßRE reduces ccn2 (ctgf) promoter activity in scleroderma fibroblasts to that seen in normal fibroblasts. Thus, the maintenance of the scleroderma phenotype, as assessed by a high degree of ccn2 (ctgf) promoter activity, appears to be relatively independent of SMAD action and seems to reflect increased basal promoter activity.

Key Words: connective tissue growth factor • scleroderma • transforming growth factor ß

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