ORIGINAL ARTICLE

Cyclin E and chromosome instability in colorectal cancer cell lines

C Simone¹, N Resta¹, L Bagella², A Giordano² and G Guanti¹

¹ Department of Internal and Public Medicine, Division of Medical Genetics, University of Bari, Bari 70124, Italy
² Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, Philadelphia, PA 19107, USA

Correspondence to:
Correspondence to:
Dr C Simone, Department of Public Medicine, Division of Medical Genetics, University of Bari, Bari 70124, Italy;
cristianosimone{at}hotmail.com

Aims/Background: The development of colorectal cancer depends on at least two distinct pathways involving genetic instability, namely: chromosome instability (CIN) and microsatellite instability. Cyclin E is involved in aneuploidy and several cancer types show an abnormal number of chromosomes.

Methods: Cyclin E protein and mRNA values were analysed in human fetal skin fibroblasts and five colorectal cancer cell lines.

Results: Cells with an aberrant number of chromosomes had higher cyclin E mRNA values and a significant increase in protein concentrations.

Conclusions: These data suggest that cyclin E regulation is altered in aneuploid cells and is an important factor in the CIN pathway.

Keywords: cyclin dependent kinase; cyclin; chromosome instability; colorectal cancer; aneuploidy

Abbreviations: cdk, cyclin dependent kinase; CIN, chromosome instability; hFSF, human fetal skin fibroblasts; MIN, microsatellite instability

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