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Epidermal expression of serine protease, neuropsin (KLK8) in normal and pathological skin samples

¹ Division of Structural Cell Biology, Nara Institute of Science and Technology, 8916–5, Takayama, Ikoma, Nara 630–0101, Japan
² Department of Plastic Surgery, Osaka University Medical School, Osaka 565-0871, Japan
³ Department of Dermatology, Osaka University Medical School
⁴ Division of Anatomy, Asahikawa Medical College, Asahikawa 078-8610, Japan

Correspondence to:
Dr S Shiosaka, Division of Structural Cell Biology, Nara Institute of Science and Technology, 8916–5, Takayama, Ikoma, Nara 630–0101, Japan;
sshiosak{at}bs.aist-nara.ac.jp

Aim: The expression of human neuropsin (KLK8) mRNA in normal and pathological skin samples was analysed and the results compared with those for tissue plasminogen activator (tPA) mRNA.

Methods: Northern blot and in situ hybridisation analyses of KLK8 mRNA in normal and lesional skin of patients with cutaneous diseases were performed.

Results: A weak signal for KLK8 mRNA and no signal for tPA mRNA was seen in normal skin on northern blot analysis. Weak signals for KLK8 were localised to the superficial cells beneath the cornified layer in normal skin on in situ hybridisation. Psoriasis vulgaris, seborrheic keratosis, lichen planus, and squamous cell carcinoma skin samples, which show severe hyperkeratosis, displayed a high density of KLK8 mRNA on northern and in situ hybridisation analyses. The signals were localised in granular and spinous layers of lesional skin in all hyperkeratic samples, including the area surrounding the horn pearls of squamous cell carcinoma. To examine the relation between mRNA expression and terminal differentiation, the expression of KLK8 mRNA was analysed in cell cultures. When keratinisation proceeded in high calcium medium, a correlative increase in the expression of KLK8 mRNA was observed.

Conclusion: The results are consistent with a role for this protease in the terminal differentiation of keratinocytes.

Keywords: neuropsin; KLK8; tissue plasminogen activator

Abbreviations: BCC, basal cell carcinoma; GAPDH, glyceralaldehyde 3-phosphate dehydrogenase; KLK, kallikrein-like; SCC, squamous cell carcinoma; tPA, tissue plasminogen activator

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