ORIGINAL ARTICLE

Further evidence to support the melanocytic origin of MDA-MB-435

G Ellison, T Klinowska, R F R Westwood, E Docter, T French and J C Fox

AstraZeneca, Mereside, Alderley Park, Macclesfield SK10 4TG, UK

Correspondence to:
Correspondence to:
Dr G Ellison, AstraZeneca, 19G22, Mereside, Alderely Park, Macclesfield SK10 4TG, UK;
gillian.ellison{at}astrazeneca.com

Background/Aims: Until recently, the cell line MDA-MB-435 was widely accepted as originating from a breast cancer. However, microarray derived data have suggested that this cell line may in fact originate from an occult melanoma. This study was designed to investigate this hypothesis further.

Methods: Quantitative reverse transcription polymerase chain reaction and immunohistochemistry were used to investigate the tissue of origin of two sublines of MDA-MB-435 (MDA-MB-435 S and MDA-MB-435 HGF). The expression of a panel of genes typical of breast cells or melanocytes was analysed.

Results: The MDA-MD-435 cell lines expressed none of the genes characteristic of breast cancer cells but did express several genes commonly expressed by melanocytes.

Conclusions: These results strongly suggest that MDA-MB-435 is indeed of melanoma origin.

Keywords: MDA-MB-435 cell line; quantitative reverse transcription polymerase chain reaction; melanoma

Abbreviations: ATCC, American Type Culture Collection; DAB, diaminobenzidine; ECACC, European Collection of Cell Cultures; EMA, epithelial membrane antigen; FCS, fetal calf serum; HGF, hepatocyte growth factor; HPRT, hypoxanthine ribosyl transferase; NEAA, non-essential amino acids; RT-PCR, reverse transcription polymerase chain reaction; SCID, severe combined immunodeficient

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