|
|
||||||||||||||
|
|
|||||||||||||||
ORIGINAL ARTICLE |
AstraZeneca, Mereside, Alderley Park, Macclesfield SK10 4TG, UK
Correspondence to:
Dr G Ellison, AstraZeneca, 19G22, Mereside, Alderely Park, Macclesfield SK10 4TG, UK;
gillian.ellison{at}astrazeneca.com
Background/Aims: Until recently, the cell line MDA-MB-435 was widely accepted as originating from a breast cancer. However, microarray derived data have suggested that this cell line may in fact originate from an occult melanoma. This study was designed to investigate this hypothesis further.
Methods: Quantitative reverse transcription polymerase chain reaction and immunohistochemistry were used to investigate the tissue of origin of two sublines of MDA-MB-435 (MDA-MB-435 S and MDA-MB-435 HGF). The expression of a panel of genes typical of breast cells or melanocytes was analysed.
Results: The MDA-MD-435 cell lines expressed none of the genes characteristic of breast cancer cells but did express several genes commonly expressed by melanocytes.
Conclusions: These results strongly suggest that MDA-MB-435 is indeed of melanoma origin.
Keywords: MDA-MB-435 cell line; quantitative reverse transcription polymerase chain reaction; melanoma
Abbreviations: ATCC, American Type Culture Collection; DAB, diaminobenzidine; ECACC, European Collection of Cell Cultures; EMA, epithelial membrane antigen; FCS, fetal calf serum; HGF, hepatocyte growth factor; HPRT, hypoxanthine ribosyl transferase; NEAA, non-essential amino acids; RT-PCR, reverse transcription polymerase chain reaction; SCID, severe combined immunodeficient
This article has been cited by other articles:
![]() |
M. Ehrich, J. Turner, P. Gibbs, L. Lipton, M. Giovanneti, C. Cantor, and D. van den Boom Cytosine methylation profiling of cancer cell lines PNAS, March 25, 2008; 105(12): 4844 - 4849. [Abstract] [Full Text] [PDF] |
||||
![]() |
P. A. Phadke, K. S. Vaidya, K. T. Nash, D. R. Hurst, and D. R. Welch BRMS1 Suppresses Breast Cancer Experimental Metastasis to Multiple Organs by Inhibiting Several Steps of the Metastatic Process Am. J. Pathol., March 1, 2008; 172(3): 809 - 817. [Abstract] [Full Text] [PDF] |
||||
![]() |
F. Shen, S. Chu, A. K. Bence, B. Bailey, X. Xue, P. A. Erickson, M. H. Montrose, W. T. Beck, and L. C. Erickson Quantitation of Doxorubicin Uptake, Efflux, and Modulation of Multidrug Resistance (MDR) in MDR Human Cancer Cells J. Pharmacol. Exp. Ther., January 1, 2008; 324(1): 95 - 102. [Abstract] [Full Text] [PDF] |
||||
![]() |
W. C. Reinhold, M. A. Reimers, A. K. Maunakea, S. Kim, S. Lababidi, U. Scherf, U. T. Shankavaram, M. S. Ziegler, C. Stewart, H. Kouros-Mehr, et al. Detailed DNA methylation profiles of the E-cadherin promoter in the NCI-60 cancer cells Mol. Cancer Ther., February 1, 2007; 6(2): 391 - 403. [Abstract] [Full Text] [PDF] |
||||
![]() |
P L Jeffery, R E Murray, A H Yeh, J F McNamara, R P Duncan, G D Francis, A C Herington, and L K Chopin Expression and function of the ghrelin axis, including a novel preproghrelin isoform, in human breast cancer tissues and cell lines Endocr. Relat. Cancer, December 1, 2005; 12(4): 839 - 850. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. G. Hollingshead, J. P. Carter, K. Dougherty, and C. A. Bonomi The Hollow Fiber Assay in Cancer Efficacy Testing in the Developmental Therapeutics Program at the National Cancer Institute Am. Assoc. Cancer Res. Educ. Book, April 1, 2005; 2005(1): 351 - 354. [Full Text] [PDF] |
||||
![]() |
R. Sandberg and I. Ernberg Assessment of tumor characteristic gene expression in cell lines using a tissue similarity index (TSI) PNAS, February 8, 2005; 102(6): 2052 - 2057. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. Moroni, V. Soldatenkov, L. Zhang, Y. Zhang, G. Stoica, E. Gehan, B. Rashidi, B. Singh, M. Ozdemirli, and S. C. Mueller Progressive Loss of Syk and Abnormal Proliferation in Breast Cancer Cells Cancer Res., October 15, 2004; 64(20): 7346 - 7354. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. C. Alley, M. G. Hollingshead, C. M. Pacula-Cox, W. R. Waud, J. A. Hartley, P. W. Howard, S. J. Gregson, D. E. Thurston, and E. A. Sausville SJG-136 (NSC 694501), A Novel Rationally Designed DNA Minor Groove Interstrand Cross-Linking Agent with Potent and Broad Spectrum Antitumor Activity: Part 2: Efficacy Evaluations Cancer Res., September 15, 2004; 64(18): 6700 - 6706. [Abstract] [Full Text] [PDF] |
||||
![]() |
R. E. Duncan, A. El-Sohemy, and M. C. Archer Mevalonate Promotes the Growth of Tumors Derived from Human Cancer Cells in Vivo and Stimulates Proliferation in Vitro with Enhanced Cyclin-dependent Kinase-2 Activity J. Biol. Chem., August 6, 2004; 279(32): 33079 - 33084. [Abstract] [Full Text] [PDF] |
||||
![]() |
Z. Liang, T. Wu, H. Lou, X. Yu, R. S. Taichman, S. K. Lau, S. Nie, J. Umbreit, and H. Shim Inhibition of Breast Cancer Metastasis by Selective Synthetic Polypeptide against CXCR4 Cancer Res., June 15, 2004; 64(12): 4302 - 4308. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. Sellappan, R. Grijalva, X. Zhou, W. Yang, M. B. Eli, G. B. Mills, and D. Yu Lineage Infidelity of MDA-MB-435 Cells: Expression of Melanocyte Proteins in a Breast Cancer Cell Line Cancer Res., May 15, 2004; 64(10): 3479 - 3485. [Abstract] [Full Text] [PDF] |
||||
![]() |
D. Sachdev, J. S. Hartell, A. V. Lee, X. Zhang, and D. Yee A Dominant Negative Type I Insulin-like Growth Factor Receptor Inhibits Metastasis of Human Cancer Cells J. Biol. Chem., February 6, 2004; 279(6): 5017 - 5024. [Abstract] [Full Text] [PDF] |
||||
![]() |
Y. Huang, J. C. Keen, E. Hager, R. Smith, A. Hacker, B. Frydman, A. L. Valasinas, V. K. Reddy, L. J. Marton, R. A. Casero Jr., et al. Regulation of Polyamine Analogue Cytotoxicity by c-Jun in Human MDA-MB-435 Cancer Cells Mol. Cancer Res., February 1, 2004; 2(2): 81 - 88. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. Kremer, L. Quintanilla-Martinez, M. Fuchs, A. Gamboa-Dominguez, S. Haye, H. Kalthoff, E. Rosivatz, C. Hermannstadter, R. Busch, H. Hofler, et al. Influence of tumor-associated E-cadherin mutations on tumorigenicity and metastasis Carcinogenesis, December 1, 2003; 24(12): 1879 - 1886. [Abstract] [Full Text] [PDF] |
||||
![]() |
Y. Huang, E. R. Hager, D. L. Phillips, V. R. Dunn, A. Hacker, B. Frydman, J. A. Kink, A. L. Valasinas, V. K. Reddy, L. J. Marton, et al. A Novel Polyamine Analog Inhibits Growth and Induces Apoptosis in Human Breast Cancer Cells Clin. Cancer Res., July 1, 2003; 9(7): 2769 - 2777. [Abstract] [Full Text] [PDF] |
||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS | REGISTER |
| Molecular Pathology | Journal of Clinical Pathology |