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Tempero–spatial dissociation between the expression of Fas and apoptosis after coronary occlusion

¹ Department of Cardiology, General Hospital of Chinese PLA, 28 Fuxing Road, Beijing 100853, China
² Department of Pathology, General Hospital of Chinese PLA
³ Department of Nephrology, General Hospital of Chinese PLA

Correspondence to:
Dr Q Z Feng
Department of Cardiology, General Hospital of Chinese PLA, 28 Fuxing Road, Beijing 100853, China; fqz301{at}yahoo.com

Aims: To explore the role of Fas in cardiomyocytic apoptosis induced by ischaemia through determining the histological relation between Fas expression and apoptosis in rat myocardium during ischaemia/infarction.

Methods: The myocardial ischaemia model was produced by ligating the left coronary artery in Sprague-Dawley rats. The rats were killed from 10 minutes to seven days after surgery. Apoptotic myocardial cells were detected by the in situ terminal deoxynucleotidyl transferase mediated nick end labelling method, and the expression of Fas by immunohistochemistry and western blotting.

Results: Cardiomyocytic apoptosis appeared from three to 36 hours after ischaemia. The expression of Fas could be detected by western blot from before surgery to seven days of ischaemia. Apoptosis and the expression of Fas in the cardiomyocytes appeared in different regions of the myocardium: apoptosis in the ischaemic region, Fas in the regions surrounding ischaemic myocardium.

Conclusion: These results suggest that there is a tempero–spatial dissociation between the expression of Fas and apoptosis after coronary occlusion. Fas might not directly regulate the apoptosis of cardiomyocytes induced by ischaemia.

Keywords: apoptosis; Fas; myocytes; heart; ischaemia; infarction

Abbreviations: FasL, Fas ligand; H&E, haematoxylin and eosin; LAD, left anterior descending branch of the left coronary artery; TdT, terminal deoxynucleotidyl transferase; TUNEL, in situ terminal deoxynucleotidyl transferase mediated nick end labelling