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Mol Path 53:150-154 doi:10.1136/mp.53.3.150

Detection of clonal T cell populations by high resolution PCR using fluorescently labelled nucleotides; evaluation using conventional LIS-SSCP

Table 1

Clonal TCRγ gene rearrangements detected in samples from patients with T cell disease using both LIS-SSCP PAGE and DNA sequencer analysis of products labelled using fluorescent nucleotides

Patient Diagnosis Clonal TCRγ gene rearrangements detected using LIS-SSCP PAGE and a DNA sequencer
LIS-SSCP PAGE, low ionic strength single strand conformation polymorphism polyacrylamide gel electrophoresis; TCR, T cell receptor.
1 Cutaneous T cell lymphoma GV1Jγ and GV2Jγ
2 Cutaneous T cell lymphoma GV1JγP and GV1JγP1/2
3 T cell non-Hodgkin's lymphoma GV3Jγ and GV4Jγ
4 T cell non-Hodgkin's lymphoma GV1Jγ and GV1JγP
5 T cell non-Hodgkin's lymphoma GV1JγP, GV2Jγ, GV2JγP1/2, GV3 JγP1/2, and GV4Jγ
6 T cell non-Hodgkin's lymphoma GV1Jγ
7 T cell non-Hodgkin's lymphoma GV1Jγ and GV2Jγ
8 Sezary syndrome GV1JγP,GV2Jγ,GV2JγP1/2,GV3JγP1/2, GV4Jγ, and GV4JγP1/2
9 T cell chronic lymphocytic leukaemia GV1Jγ
10 T cell prolymphocytic leukaemia GV2Jγ and GV3Jγ
11 T cell γδ hepatosplenic lymphoma GV1Jγ

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