Table 2
Primers and probes used for HHV-8 vIL-6, v-cyclin, and GPCR PCR
| Primer/probe | Sequence |
|---|---|
| F, FAM; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GPCR, G protein coupled receptor; HHV-8, human herpesvirus 8; T, TAMRA; V, VIC; v-IL-6, viral interleukin 6. | |
| v-Cyclin | |
| Forward primer | 5′-GCC GCC TGT AGA ACG GAA A-3′ |
| Reverse primer | 5′-GCT CAT TGC CCG CCT CTA-3′ |
| Probe | 5′-F-CAG GAA CCA ACA GCG CAC AGC C-T-3′ |
| v-IL-6 | |
| Forward primer | 5′-CTC TTG CTG GTC GGT TCA CTG-3′ |
| Reverse primer | 5′-TGC CGG TAC GGT AAC AGA GG-3′ |
| Probe | 5′-F-CGG ACG CCC CCG AGT TTG AAA A-T-3′ |
| GPCR | |
| Forward primer | 5′-GTG GTG CCT TAC ACG TGG AA-3′ |
| Reverse primer | 5′-GCG AGT TTA GGC AGA TAC CCA G-3′ |
| Probe | 5′-F-TTT TTG CAA GCA CCG ATC GCG GG-T-3′ |
| GAPDH | |
| Forward primer | 5′-GAA GGT GAA CGG AGT-3′ |
| Reverse primer | 5′-GAA GAT GGT GAT GGG ATT TC-3′ |
| Probe | 5′-V-CAA GCT TCC CGT TCT CAG CC-T-3′ |
