Table 2
Comparison of amplification crossing points, calculated values for DNA and RNA concentration, and relative percentage recoveries for the kits using nucleic acid extracted by the high pure nucleic acid kit as the standard
| Virus | Extraction kit | Noise band crossing point | Calculated copies/ml (millions) | Relative recovery |
|---|---|---|---|---|
| HBV, hepatitis B virus; HCV, hepatitis C virus. | ||||
| HBV | High Pure viral nucleic acid | 12.8 | 23.3 | 100% |
| QIAamp viral RNA | 18.99 | 0.5 | 2.2% | |
| QIAamp blood DNA | 13.25 | 17.7 | 76% | |
| NucleoSpin virus | 16.69 | 2.2 | 9.3% | |
| High Pure viral RNA | 17.41 | 1.4 | 6.0% | |
| HCV | High Pure viral nucleic acid | 28.03 | 2.5 | 100% |
| QIAamp viral RNA | 28.8 | 1.6 | 64% | |
| QIAamp blood DNA | 28.23 | 2.2 | 89% | |
| NucleoSpin virus | 36.23 | 0.02 | 0.9% | |
| High Pure viral RNA | 29.36 | 1.2 | 46% | |
