Table 2
TRANSFAC analysis identified several potential transcription factor binding sites in the 600 bp fragment (−559 to +39) of the ccn2 (ctgf) promoter
| Position | Transcription factor binding site | Core similarity (%) | Matrix similarity (%) |
|---|---|---|---|
| Default settings of the Matinspector professional software were used (core similarity, 0.85; matrix similarity, 0.75). Only potential transcription factor binding sites with a core similarity of 1 are shown. | |||
| *Matrix similarity in MZF1 in the GG genotype. | |||
| †GC genotype. | |||
| ‡Pbx-1 matrix similarity in CC genotype. | |||
| ¶In GC genotype. | |||
| −524 to −521 | MBP-1/RFX1 complex | 100 | 75.3 |
| −473 to −470 | TATA box | 100 | 85.1 |
| −467 to −462 | Oct1 | 100 | 86.0 |
| −467 to −462 | GFI1 | 100 | 97.1 |
| −444 to −441 | MZF1 | 100 | 98.6*, 98.02† |
| −418 to −415 | Ikaros 3 | 100 | 89.4 |
| −418 to −415 | STAT 1 | 100 | 89.2 |
| −384 to −381 | STAT 1 | 100 | 89.2 |
| −319 to −316 | S8 | 100 | 96.2 |
| −319 to −316 | Homeo domain factor Nkx-2.5/Cxs, tinman | 100 | 88.2 |
| −307 to −304 | HNF-3/homolog 8 | 100 | 95.4 |
| −307 to −303 | FREAC 4 | 100 | 79.3 |
| −306 to −303 | HNF-3/homolog 1 | 100 | 85.3 |
| −304 to −301 | TATA box | 100 | 91.9 |
| −301 to −299 | FREAC 2 | 100 | 89.1 |
| −288 to −285 | NF-E2 p45 | 100 | 86.9 |
| −248 to −245 | E2F | 100 | 84.9 |
| −219 to −216 | NFAT | 100 | 98.3 |
| −174 to −167 | SMAD binding element | 100 | Ref 21 |
| −156 to −144 | TGFβ responsive element | 100 | Ref 20 |
| −152 to −149 | ARP1 | 100 | 89.4 |
| −145 to −142 | MEF3 | 100 | 83.4 |
| −141 to −138 | Cut-like homeo domain protein | 100 | 97.5 |
| −138 to −135 | Pbx-1 | 100 | 82.1‡, 81.8¶ |
| −106 to −103 | MZF1 | 100 | 99.5 |
| −92 to −89 | c-Rel | 100 | 96.1 |
| −92 to −89 | NFκB p65 | 100 | 89.7 |
| −86 to −82 | NFκB | 100 | 92.5 |
| −82 to −79 | HNF-3/homolog 8 | 100 | 90.5 |
| −56 to −52 | CCAAT box | 100 | 91.5 |
| −55 to −41 | Nuclear factor Y box | 100 | 94.9 |
| −29 to −23 | TATA box | 100 | 99.0 |
| −10 to −7 | SP-1 | 100 | 91.4 |
| −10 to –7 | RRE | 100 | 86.2 |
| −8 to –5 | E2F | 100 | 84.9 |
| 15 to 18 | GABP | 100 | 88.7 |
| 15 to 18 | Elk-1 | 100 | 91.7 |
| 15 to 18 | NRF-2 | 100 | 95.6 |
