Table 1
Biochemical profiles and identification of the blood culture isolate by conventional biochemical tests, the Vitek GPI system, and the API Staph system
| Biochemical reactions/enzymes | Conventional | Vitek GPI | API Staph |
|---|---|---|---|
| Catalase | + | + | |
| Tube coagulase | − | − | |
| Slide coagulase | + | ||
| Resistance to bacitracin | − | − | |
| Resistance to optochin | + | + | |
| Growth in 6% NaCl | + | + | |
| Growth in 10% bile | + | ||
| Growth in 40% bile | + | + | |
| Esculin hydrolysis | − | − | |
| Arginine hydrolysis | + | + | + |
| Ornithine decarboxylase | − | ||
| Urease | + | + | + |
| Tetrazolium reduction | + | ||
| Resistance to novobiocin | − | − | |
| Resistance to polymyxin B | + | ||
| Utilisation of | |||
| Hemicellulase | + | ||
| Dextrose | + | ||
| Lactose | + | + | + |
| Mannitol | + | + | + |
| Raffinose | − | − | − |
| Salicin | − | − | |
| Sorbitol | − | − | |
| Sucrose | + | + | + |
| Trehalose | + | + | + |
| Arabinose | − | − | |
| Pyruvate | + | + | |
| Pullulan | − | ||
| Inulin | − | − | |
| Melibiose | − | − | − |
| Melezitose | − | ||
| Cellobiose | − | − | |
| Ribose | − | − | |
| Xylose | − | − | − |
| D-glucose | + | + | |
| D-fructose | + | + | |
| D-mannose | + | + | |
| Maltose | − | + | |
| Xylitol | − | ||
| α-methyl-D-glucoside | − | ||
| N-acetyl-glucosamine | − | ||
| Nitrate reduction | + | + | |
| Alkaline phosphatase | + | ||
| Identification | Staphylococcus species | S simulans 94% | S aureus 86.8% |
| S haemolyticus 3% | S warneri 5.1% |
