rss
Mol Path 55:121-124 doi:10.1136/mp.55.2.121
  • Technical report

Improved technique for fluorescence in situ hybridisation analysis of isolated nuclei from archival, B5 or formalin fixed, paraffin wax embedded tissue

Table 1

Mean number and percentage of hybridisation signals for each isolated nucleus in blood control, B5 fixed, and formalin fixed nuclei achieved with the X centromere specific probe

Mean number (%) of cells with the indicated number of hybridisation signals
Specimen Fixative Number of nuclei 0 1 2 3 4+
Blood Carnoy's 200 5 (2.5) 186 (93.0) 7 (3.5) 2 (1.0)
Tonsil B5 200 3 (1.5) 186 (93.0) 8 (4.0) 3 (1.5)
Tonsil Formalin 200 6 (3.0) 187 (93.5) 7 (3.5)
MCL B5 100 2 (2.0) 76 (76.0) 18 (18.0) 4 (4.0)

This Article

  • Pathology jobs

    Pathology jobs