The assessment of DNA and RNA preservation in liquid based cytology media
- Department of Pathology, UPCM UPR2163 CNRS, CHU Purpan, Avenue de Grande Bretagne, 31059 Toulouse Cedex; brousset.p@chu-toulouse.fr
The Papanicolaou cervical smear method has long been used for cervical cancer screening, but there is a trend to develop liquid based cytology media and monolayer techniques to reduce false negative rates. These last two types of technique seem to increase the sensitivity of cervical cancer detection,1 and also provide cellular residues that are useful either for spotting additional slices or performing molecular techniques, such as hybrid capture or the polymerase chain reaction (PCR).2 Different media exist and to date the PreservCyt™ medium used in the ThinPrep™ Pap Test™ method (Cytyc, Boxborough, Massachusetts, USA) has been validated by the …
