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Mol Path 55:156-163 doi:10.1136/mp.55.3.156
  • Original Article

Limitations of cytokeratin 20 RT-PCR to detect disseminated tumour cells in blood and bone marrow of patients with colorectal cancer: expression in controls and downregulation in tumour tissue

Table 6

Cytokeratin 20 (CK20) mRNA and protein expression, measured by means of immunohistochemical staining with antibody Ks 20.10, in colorectal tumour samples

CK20 expression
Protein
No. Tumour cells (%) RNA Positive cells Intensity
No. refers to the corresponding number in fig 2. mRNA: 1, positive after 30 cycles; 2, positive after 27 cycles; 3, positive after 24 cycles; 4, positive after 21 cycles. Positive cells: +, <25% positive; ++, 25–75% positive; +++, >75% positive. Intensity: +, weak staining; ++, moderate staining; +++, strong staining.
1 40 2
2 80 3 +++ +++
3 30 4 +++ +++
4 70 4
5 60 3 +++ +++
6 70 3 +++ ++
7 50 3 +++ +++
8 50 3 +++ +++
9 70 3 + +++
10 70 3 ++ +
11 80 3 +++ ++
12 70 3 +++ +++
13 40 1 + +++
14 60 3 ++ +++
15 20 3 +++ ++
16 60 3 ++ ++
17 50 3 +++ +++
18 80 3 ++ +++

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