Table 3
Optimised ARMS conditions for 11 tumours and the T47D cell line with a p53 mutation
| Patient/cell line | 3′ mismatch | 2nd mismatch | Product size | Ta (°C) | Mg conc. (mM) | No. of PCR cycles |
|---|---|---|---|---|---|---|
| *ARMS failed to optimise. | ||||||
| ARMS, amplification refractory mutation system; Ta, annealing temperature. | ||||||
| T47D | T–G | A–A | 127 bp | 64 | 1.75 | 35 |
| B3 | A–G | A–A | 87 bp | 64 | 2 | 35 |
| B11 | G–T | C–T | 140 bp | 67 | 1.5 | 35 |
| B16 | G–G | C–C | 130 bp | 64 | 2 | 35 |
| B27 | A–C | A–G | 143 bp | 60 | 2 | 35 |
| B28* | G–T | G–G | 78 bp | |||
| B30 | T–C | C–C | 115 bp | 56 | 2 | 35 |
| B33* | G–T | G–G | 78 bp | |||
| B37 | A–C | C–T | 112 bp | 69 | 1.75 | 33 |
| B45 | T–G | C–C | 125 bp | 60 | 1.75 | 35 |
| B46* | A–G | C–T | 170 bp | |||
| B50* | G–T | T–C | 170 bp | |||
