Cyclin E and chromosome instability in colorectal cancer cell lines
- 1Department of Internal and Public Medicine, Division of Medical Genetics, University of Bari, Bari 70124, Italy
- 2Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, Philadelphia, PA 19107, USA
- Correspondence to: Dr C Simone, Department of Public Medicine, Division of Medical Genetics, University of Bari, Bari 70124, Italy; cristianosimone{at}hotmail.com
- Accepted 13 December 2001
Abstract
Aims/Background: The development of colorectal cancer depends on at least two distinct pathways involving genetic instability, namely: chromosome instability (CIN) and microsatellite instability. Cyclin E is involved in aneuploidy and several cancer types show an abnormal number of chromosomes.
Methods: Cyclin E protein and mRNA values were analysed in human fetal skin fibroblasts and five colorectal cancer cell lines.
Results: Cells with an aberrant number of chromosomes had higher cyclin E mRNA values and a significant increase in protein concentrations.
Conclusions: These data suggest that cyclin E regulation is altered in aneuploid cells and is an important factor in the CIN pathway.
- cdk, cyclin dependent kinase
- CIN, chromosome instability
- hFSF, human fetal skin fibroblasts
- MIN, microsatellite instability
