Table 2
Inactivation of creatine kinase (CK) and phosphoglycerate mutase (PGM) activity by urate
| CK | PGM | |||
|---|---|---|---|---|
| No urate | Plus urate | No urate | Plus urate | |
| Purified PGM and CK from rabbit muscle (3.8 mg/ml each) and human muscle extract (0.4 U/ml), previously reactivated by incubation with 10mM dithiothreitol (DTT) for 30 minutes at 30°C, were incubated at 25°C in buffer (50mM Tris/HCl, pH 7.4) in the presence and in the absence of 4mM urate. After 24 hours of incubation, the activities of PGM and CK were assayed directly and after a further 30 minutes of incubation at 30°C with 10mM DTT (numbers in parenthesis). | ||||
| Human | 100 | 45 (71) | 77 | 80 |
| Rabbit | 88 (88) | 38 (59) | 90 (87) | 82 (82) |
