Table 1
Primers used in the reverse transcription-polymerase chain reaction protocol designed to detect melanoma metastases and their product size
| cDNA target | Primer | Sequence from 5′ to 3′ end | Nucleotide position (exon number) | Product size (bp) | Ref |
|---|---|---|---|---|---|
| Nucleotide positions are based on the sequences with the following GenBank accession numbers: Y00819 for tyrosinase, U06654 for MART-1, M77348 for Pmel-17, U03735 for MAGE-3, and NM_000190 for PBGD. The exon information for tyrosinase is based on GenBank accession numbers AF237808 and AF237809; for MART-1 on U06654; for Pmel-17 on U31797, U31798, U31799, U31807, and U31808; for MAGE-3 on U03735; and for PBGD on M18799, M18800, D12722, X68018, and X04217. | |||||
| *Unpublished forward primer for Pmel-17, designed by Hanekom and co-workers using DNAMAN 5.1.0.0 (Lynnon Biosoft). | |||||
| PBGD, porphobilinogen deaminase. | |||||
| Tyrosinase | >Htyr3 | GTC TTT ATG CAA TGG AAC GC | 864–883 (2) | 207 | 33 |
| <Htyr2 | AGG CAT TGT GCA TGC TGC TT | 1083–1102 (3) | |||
| MART-1 | >MART-1 | CAC TCT TAC ACC ACG GCT GA | 215–234 (2) | 299 | 33 |
| <MART-1 | AGG TGA ATA AGG TGG TGG TGA | 494–514 (5) | |||
| Pmel-17 | >Pmel-17* | TGC TGG AGA GGT GGT CAA GTG | 189–209 (2/3) | 699 | 29 |
| <Pmel-17 | CTC CAG GTA AGT ATG AGT GAC | 837–857 (6) | |||
| MAGE-3 | >MAGE-3 | GAA GCC GGC CCA GGC TCG | 438–455 (1) | 413 | 34 |
| <MAGE-3 | GGA GTC CTC ATA GGA TTG GCT CC | 2703–2725 (3) | |||
| PBGD | >PBGD | CTG GTA ACG GCA ATG CGG CT | 32–51 (1) | 339 | 33 |
| <PBGD | GCA GAT GGC TCC GAT GGT GA | 350–369 (5) | |||
