Housekeeping genes confound mRNA assays in asthma
A n Australian study is the first to question routine use in asthma research of two genes widely used for standardising quantitative mRNA assays. The expression of β actin and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) needs careful validation first, say the researchers.
They used a reverse transcriptase (RT)-PCR method that does not …
